| Abstract： |
Excessive heavy metals (HMs) in livestock manure due to additives over-use limits its recycling through composting. However, few studies have investigated the interactive influence of microbial communities, functional genes, and environmental factors in HM immobilization during composting. Therefore, treatments with different C/N ratios (15:1, 20:1, 25:1) and bulking agents (maize straw, green waste, vinasse) were conducted to explore the HMs immobilization pathways using structural equation model (SEM). Results confirmed the promoting effect of C/N ratio of 20:1 and vinasse on microbial diversity, thus leading to greater HMs immobilization rate. Meanwhile, the dominant microbial phyla of Cu/Zn, Cd, As, and Cr were identified as Proteobacteria, Firmicutes, Chytridiomycota, and Bacteroidota, respectively. Moreover, the significant correlation between functional genes (copC, mt, cbhl, aoxB, yieF) and HMs illustrated potential immobilization effects of metal-binding proteins on Cu and Zn, humus complexation on Zn, and oxidase/reductase on As/Cr. Finally, SEM indicated that the redistribution of Cu, Zn, As, and Cr fractions was interactively influenced by environmental factors (organic matter, pH, electrical conductivity, and total phosphorus), microbial communities, and functional genes, while Cd was directly regulated by organic matter and total phosphorus. These results may provide a deeper understanding of HM immobilization pathways during swine manure composting. |
